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Schriftzug Angewandte Genetik Collage von Motiven der Angewandten Genetik

Dahlem Research School

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Home Page » Research groups » Heyl » Transcriptional regulation

Research group Heyl

Transcriptional regulation

The response to cytokinin is mediated in large part by alterations in the transcriptome. These changes in transcript level are regulated by transcription factors. The most important group of transcriptional regulators of the cytokinin response are the type-B response regulators. Functional analysis of knock-out mutants of the different type-B response regulators is hampered by the high level of redundancy among the eleven members of this protein family. In order to circumvent this problem, we generated a dominant repressor of the type-B ARRs by fusing the SRDX repressor domain to ARR1, one of the type-B ARRs, and expressing it under the control of a 35S promoter. The resulting plants showed all the hallmarks of the cytokinin deficiency syndrome (Heyl et al., 2008; Figure2A). Phenotypic and molecular analysis of the resulting plants indicated that most if not all of the cytokinin response is mediated via this class of transcription factors (Figure 2B, C). However, promoter deletion experiments showed that additional factors are involved in fine-tuning of the transcriptional response to cytokinin (Ramireddy et al, 2013). In addition several genetic screens identified several putative co-regulators of this signaling pathway and these are currently under investigation.

Phenotype of B-type ARR dominant repressor plants
Altered phenotype of B-type ARR dominant repressor (35S::ARR1-SRDX) plants. (A) ARR1-SRDX plants (on the right) show a severely stunted phenotype when compared to the wild-type (left). (B) Bioassays for shoot and root show strongly decreased cytokinin sensitivity. (C) Expression of cytokinin primary response genes is clearly dampened in dominant repressor plants (Heyl et al., 2008).

News

New publication

An article about the analysis of a cytokinin-responsive gene of Arabidopsis thaliana encoding a novel F-box protein regulating sterol biosynthesis has been published in Journal of Experimental Botany.
May 2017

New publication

A report about constitutively active mutants of the cytokinin receptors AHK2 und AHK3 and their impact on organ size, flowering time, and plant longevity has been published in Plant Physiology.
March 2017

New publication

An article about the role of the transcription factor ERF105 in freezing tolerance and cold acclimation has been published in Plant Cell and Environment.
January 2017

Krolow Award 2016

The Krolow Award 2016 was given to Dr. Anne Cortleven for her work about the role of Cytokinin in the plant response to high light stress.
November 2016
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Updated: 2017-04-23